Cell Culture & Passaging
Maintenance, thawing, cryopreservation, and passaging of diverse cell lines.
Experience with: pancreatic cancer cell lines (e.g. PANC-1, Mia PaCa-2, AsPC-1, BxPC-3), primary fibroblasts, and immune cell lines (e.g. T cells, THP-1).
Cell Proliferation Assay (CCK-8, EdU, Colony Formation)
Assessment of cell viability, proliferation and long-term clonogenic ability using CCK-8, EdU incorporation, and colony formation assays.
Migration & Invasion Assay (Transwell, Wound Healing)
Evaluation of cell motility and invasion using transwell migration/invasion, and scratch (wound healing) assays.
RNA Extraction & qRT-PCR
RNA isolation, cDNA synthesis, and quantitative real-time PCR; primer design and data normalization.
Protein Extraction & Western Blot
Lysis, quantification, SDS-PAGE, PVDF transfer, antibody incubation, and chemiluminescent detection.
mRNA Stability Detection
Assessment of mRNA half-life after transcription inhibition by Actinomycin D treatment.
Protein Stability Detection
Cycloheximide chase assay to monitor protein degradation rates.
Protein Ubiquitination Detection
Proteasome inhibitor (MG132) treatment to detect ubiquitinated proteins by Western blot.
RNA-Protein Interaction Assays (RIP, MeRIP, CHIRP)
Identification of RNA-binding proteins or modified RNAs using immunoprecipitation and related techniques.
Polysome Profiling
Sucrose gradient separation to analyze mRNA translation efficiency.
Liquid Chromatography-Mass Spectrometry (LC-MS)
Analysis of proteins and peptides by LC-MS for identification and quantification.
RNA-FISH
Fluorescence in situ hybridization for subcellular localization of RNA molecules.
Immunofluorescence Staining (IF)
Fluorescent labeling and confocal imaging for protein/cellular markers.
Immunohistochemistry (IHC)
Protein expression in tissue sections using antibody-based labeling.
Enzyme-linked Immunosorbent Assay (ELISA)
Quantitative measurement of secreted proteins in media/serum.
Flow Cytometry
Immunophenotyping, intracellular cytokine detection, and quantitative immune cell analysis (FlowJo).
LDH Release Assay
Measurement of cytotoxicity in tumor-immune co-cultures.
T cell-mediated Cytotoxicity Assay
Co-culture with Jurkat T cells to assess immune killing and effector molecule expression.
Bulk RNA-seq Analysis (R)
DESeq2 for differential expression, ClusterProfiler, GSEA, pathway analysis.
Single-cell RNA-seq Analysis (Python)
Preprocessing, batch correction, clustering, annotation, visualization (Scanpy, Seurat, scvi-tools).
Statistical Analysis (R, GraphPad, Python)
Survival analysis (Kaplan–Meier, Cox regression), correlation, data visualization (ggplot2, Prism).
Image Processing (ImageJ, GraphPad Prism)
Quantification of microscopy images, Western blots, general plotting.